close
1.

論文(リポジトリ)

論文(リポジトリ)
山本, 洋子
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  112  pp.552-562,  1998-09.  新潟医学会
本文リンク: http://hdl.handle.net/10191/46200
概要: To investigate the effect of epidermal growth factor (EGF) on cell migration of squamous cell carcinoma (SCC) cells, mig ration, adhesion and spreading activity, organization of focal adhesion and actin stress fiber, integrin expression, and tyrosin phosphorylation of focal adhesion kinase (FAK) of a cell line were examined on type IV collagen in the presence of various concentrations of EGF. The cell line that was separated from an established cell line of SCC in uterine cervix, CaSki, expresses α2, α3, α6, β1 and β4 integrins, and was used in this study. Wound assay demonstrated that EGF increased the migration activity at 1 and 10 ng/ml, whereas it completely blocked the migration at 100 ng/ml. Blocking antibodies against α3, α6 and β1 integrins inhibited the migration in the absence of EGF, whereas antibodies against not only these integrins but also α2 inhibited it in the presence of 10 ng/ml EGF. Flowcytometry showed that EGF increased the expression of α2 and α6 integrins in a dose dependent manner. Attachment assay revealed that EGF decreased the adhesion activity to type IV collagen in a dose dependent manner. Morphological examinations showed that EGF inhibited the spreading at 10 and 100 ng/ml, and that the growth factor did not alter the organization of focal adhesions and actin stress fibers at 1 and 10 ng/ml, whereas it increased it at 100 ng/ml. Immunoprecipitation and immunoblot assays showed that EGF did not alter tyrosin phosphorylation level of FAK at any concentrations examined. These findings suggest that the EGF-mediated promotion of the migration at 1 and 10 ng/ml is related with the modulation of α2 integrin function and expression, and the inhibition of the adhesion, whereas EGF-mediated inhibition of the migration is related with the modulation of organization of actin stress fiber and focal adhesion, and that alteration in a tyrosin phosphorylation level of FAK is not always accompanied with an increase in the migration activity. 続きを見る
2.

論文(リポジトリ)

論文(リポジトリ)
兼子, 泰行
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  105  pp.177-186,  1991-03.  新潟医学会
本文リンク: http://hdl.handle.net/10191/38651
概要: To investigate alterations in the basement membrane (BM) and of fibronectin receptor (FNR) in malignant melanoma (MM), t he tumor were studied immunohistochemically by double immunofluorescent staining using monoclonal antibodies to the core protein of heparan sulfate proteoglycan(HSPG), collagen type III(C-3)and chondroitin 6-sulfate (C6S) as well as antiserum to laminin, collagen type IV (C-4), fibronectin and fibronectin receptor. In MM in situ, C-4, laminin and HSPG were preserved as a continuous linear band on the dermo-epidermal junction (DEJ), while C6S is extensively lost from the DEJ and fibronectin is remarkably decreased in the papillary dermis compared with the normal skin. In invasive, MM, although the distribution pattern was varied case by case, the BM components were not decreased. The cases were divided into two groups by the distribution pattern; a great amount of each BM component were localized on the surface of tumor cells, or surrounded tumor nests, forming the large nests. In all but one cases examined, however, C6S was absent from the BM zone of the melanoma nests in the dermis. These data indicates that although C6S was absent, the other BM components were actively synthesized by tumor cells, and that the BM components were altered in phase of MM in situ. Intensity of FNR of melanoma cells were generally more fainter than those of infiltrating lymphocytes in the dermis, basal cells of the epidermis and endothelial cells of small vessels. There were no significant differences in their intensity of melanoma cells between each tumor progression phase. Therefore, the present study may reveal that these receptors do not relate to invasion or metastasis of the tumor. 続きを見る
3.

論文(リポジトリ)

論文(リポジトリ)
村瀬, 真一
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  123  pp.1-8,  2009-01.  新潟医学会
本文リンク: http://hdl.handle.net/10191/28226
概要: ヒトをはじめ哺乳類の脳には, その発達期だけでなく生涯を通じて側脳室周囲から嗅球へ向かいニューロブラスト (神経前駆細胞) が一方向性に移動する経路があり rostral migratorystream (HMS) と呼ばれている. RMS は, 側脳室周囲のsubventricular zone (SVZ) と呼ばれる領域に起こり嗅球の中央に終わる. RMSを移動するニューロブラストは, 嗅球へ到達するとニューロンへと分化する. この移動と分化に必要な因子の同定は神経発達メカニズムの理解に重要なだけでなく, 神経再生に応用出来る可能性がある重要な課題である. RMSのニューロブラスト研究の歴史を概説するとともに, ニューロブラストに発現する接着因子に着冒して, その移動と分化の制御機構を明らかにしようと考えている私達の研究を紹介する. 続きを見る