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1.

論文(リポジトリ)

論文(リポジトリ)
森山, 美昭
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  110  pp.311-315,  1996-08.  新潟医学会
本文リンク: http://hdl.handle.net/10191/43251
概要: In order to improve the efficiency of gene transduction into human hematopoietic stem cells by retrovirus vectors, we conducted an in vitro study to determine the optimal conditions. Cells used as the target were CD34-enriched human bone marrow stem cells. Retrovirus vectors used were LNL6 and G1Na40, both carrying the Neomycin-resistant (Neo-R) gene as a genetic marker. Transduction efficiency varied from 5.1% to 35.0%, depending on the factors and procedures in the experiments, which cornprised MOI, the duration of exposure of cells to the vectors, and conduct/nonconduct of centrifuge of the cells during the exposure. The most effective gene transduction into CFV-GM was found to be a 48h-liquid culture with growth factors followed by 48h-vector exposure with the spin transduction. 続きを見る
2.

論文(リポジトリ)

論文(リポジトリ)
宮路, 智香子
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  112  pp.671-675,  1998-11.  新潟医学会
本文リンク: http://hdl.handle.net/10191/46333
概要: Recent studies demonstrated that murine liver contained extrathymically differentiated T cells. These cells express inte rmediate (int) level of TCR (CD3) and IL-2Rβ chain. However, it has been unknown about the development of these int TCR cells without requirement of thymus. Here, we investigated how the int TCR cells were differentiated and proliferated. The int TCR cells but not the other peripheral T cells responded to exogenous IL-7 which supported TCR cell differentiation in thymus. Similar to the bone marrow (BM), the liver contained hematopoietic stem cells (c-kit^+Lin^-) which enabled to reconstitute T, B, macrophage and myeloid cells in irradiated SCID mice. In vitro, the hematopoietic stem cells in the liver did not differentiate into the int TCR cells either in the presence of fetal thymus or thymic stromal cells. However liver-derived stromal cells which could produce IL-7 and other cytokines, supported int TCR cell differentiation. On the other hand, those in BM did not develop the int TCR cells in any condition. Taken together, the int TCR cells in the liver are supposed to be developed in situ from the hematopoietic stem cells in the liver in association with some cytokines like IL-7. 続きを見る
3.

論文(リポジトリ)

論文(リポジトリ)
Moriyama, Yoshiaki ; Takahashi, Masahiro ; Masuko, Masayoshi ; Kishi, Kenji ; Shibata, Akira
出版情報: Acta medica et biologica = Acta medica et biologica.  43  pp.217-222,  1995-12.  Niigata University School of Medicine
本文リンク: http://hdl.handle.net/10191/33220
概要: In order to improve the efficiency of gene transduction into human hematopoietic stem cells by retrovirus vectors, we conducted an in vitro study to determine the optimal conditions. Cells employed as the target were K562, a human myeloblastoid cell line. Retrovirus vectors used were LNL6 and GINa40, both carrying the Neo^R (Neomycin-resistant) gene as a genetic marker. LNL6 was provided by Genetic Therapy Inc. (Gaithersburg, MD, U.S.A.) as culture supernatants of the producing cells; GINa40 was of the supernatants alike and their concentrates from the same Inc. Transduction efficiency varied from 1.0% to 59.1% depending upon the factors and procedures in the experiments, which comprised MOI (multiplicity of infection) values, the duration of exposure of cells to the vectors, and conduct/non-conduct of centrifuge of cells during the exposure. An optimal transduction was achieved by daily supplementation up to three days of the vectors to cells, together with a centrifuge of the cells at 2,500 rpm for 90 min during their exposure to the vectors. Along with the Neo^R gene transduction, a new assay system was introduced as a related matter of importance. 続きを見る