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1.

論文(リポジトリ)

論文(リポジトリ)
羽生, 忠正
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  112  pp.371-379,  1998-07.  新潟医学会
本文リンク: http://hdl.handle.net/10191/45971
概要: To study joint destruction and osteoporosis in patients with rheumatoid arthritis (RA), we investigated bone loss using histomorphometric analysis. We also examined the property of colony forming unit-fibroblastic (CFU-F) isolated from tibial bone marrow in rats with collagen-induced arthritis (CIA) and iliac bone marrow in human. Our findings showed decreases of parameters of bone formation and of the number of alkaline phosphatase-positive CFU-F during onset of arthritis. We speculated that reduced bone formation was the predominant mechanism of bone loss in the pathogenesis of RA. We also demonstrated that the lymphocytes in the joints of CIA comprised resident T cells that are extrathymically generated in situ. In vivo treatment with various monoclonal antibody, these γδT cells and CD 8 _<αα>^+ cells were associated with suppression of the disease, especially after its onset. On the other hand, in patients with RA, CD57^+T cells levels were elevated, especially high in joints and its adjacent bone marrow. CD57^+T cells contained higher proportions of CD ^4-^8- cells and γδT cells than CD57^-T cells. We suggested that CD57^+T cells with NK cell marker probably were a counterpart of extrathymic T cells in human and had unique immuno-suppressive functions. In rats with CIA, great increase in number of granulocytes were also observed before the onset of arthritis. From these results, we are performing the evaluation of these treatment which makes it possible to decrease the granulocytes or to increase the CD57^+T cells using previous analysis system of bone formation. 続きを見る
2.

論文(リポジトリ)

論文(リポジトリ)
橋本, 誠雄
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  108  pp.358-366,  1994-05.  新潟医学会
本文リンク: http://hdl.handle.net/10191/38259
概要: In a series of recent studies, extrathymic T cell differentiation in mice has been reported. These T cells may be more p rimitive than that of thymic origin. Since they carry self reactive forbidden clones, it could be benefical for elimenating their own atypical cells. They express IL-2Rβ similar to NK cells, containing CD4 or CD8 double negative cells. Their morphology is large granular lymphocyte (LGL). This study is to elucidate the possibility that CD56 positive T cells with a morphology of LGL are the counterpart of extrathymic T cells in human. CD56 positive T cells were abundant in the liver comparing in peripheral blood from same donor. The phenotype of T cells in the liver mononuclear cells (MNC), CD56 positive T cells had considerably higher proportion of TCR-γδ aquisition, and CD4, CD8 double negative population were also existed in one of the case who had high population of CD56 positive T cells. While CD56 positive cells were absent in the thymus. CD56 positive T cells were able to proliferate in serum-rich medium condition supplemented with IL-2. CD56 positive T cells were also harvested from bone marrow cells in a liquid culture without thymic environment. These results described above and similarities of the extrathymic T cells in mice, strongly indicate the possibility that CD56 positive T cells is a counterpart of extrathymic T cells in humans. 続きを見る
3.

論文(リポジトリ)

論文(リポジトリ)
安保, 徹
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  108  pp.173-177,  1994-03.  新潟医学会
本文リンク: http://hdl.handle.net/10191/38110
概要: Genetic background and microenvironmental factors are highly responsible for the onset of autoimmune diseases. However, the effector lymphocytes, which attack directly the living tissues, are also an important factor to think about the mechanisms involved in the onset of diseases. How these effector lymphocytes increase in the number in patients with autoimmune diseases, and what kinds of properties in these cells lead to the onset of diseases? To date, extrathymic T cells and CD5^+B cells are focused attention as such the effector cells. Herein, these cells will be dealed as a central subject to be introduced, and the mechanisms involved in the onset of diseases will be discussed. 続きを見る
4.

論文(リポジトリ)

論文(リポジトリ)
宮路, 智香子
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  112  pp.671-675,  1998-11.  新潟医学会
本文リンク: http://hdl.handle.net/10191/46333
概要: Recent studies demonstrated that murine liver contained extrathymically differentiated T cells. These cells express inte rmediate (int) level of TCR (CD3) and IL-2Rβ chain. However, it has been unknown about the development of these int TCR cells without requirement of thymus. Here, we investigated how the int TCR cells were differentiated and proliferated. The int TCR cells but not the other peripheral T cells responded to exogenous IL-7 which supported TCR cell differentiation in thymus. Similar to the bone marrow (BM), the liver contained hematopoietic stem cells (c-kit^+Lin^-) which enabled to reconstitute T, B, macrophage and myeloid cells in irradiated SCID mice. In vitro, the hematopoietic stem cells in the liver did not differentiate into the int TCR cells either in the presence of fetal thymus or thymic stromal cells. However liver-derived stromal cells which could produce IL-7 and other cytokines, supported int TCR cell differentiation. On the other hand, those in BM did not develop the int TCR cells in any condition. Taken together, the int TCR cells in the liver are supposed to be developed in situ from the hematopoietic stem cells in the liver in association with some cytokines like IL-7. 続きを見る
5.

論文(リポジトリ)

論文(リポジトリ)
Yamamura, Soichiro ; Miyaji, Chikako ; Watanabe, Hisami ; Kawamura, Toshihiko ; Fukuda, Minoru ; Abo, Toru
出版情報: Acta medica et biologica = Acta medica et biologica.  44  pp.177-185,  1996-12.  Niigata University School of Medicine
本文リンク: http://hdl.handle.net/10191/33179
概要: Some traditional Chinese medicines are known to have paradoxical effects. For example, one such medicine (i.e., glycyrrhizin) is effective for the treatment of allergic diseases (i.e., lymphocyte-associated inflammation), and is also sometimes effective for the treatment of suppurative diseases (i.e., granulocyte-associated inflammation). To investigate the mechanisms underlying this paradox, we conducted a study in mice. When normal mice were administered with one i. p. injection of glycyrrhizin, granulocytes and extrathymic T cells increased in proportion and number in various immune organs. However, an opposite response of glycyrrhizin was induced with such an injection in mice with preexisting granulocytosis. These mice included those treated with G-CSF and AIDS mice infected with murine leukemia virus. In other words, glycyrrhizin reduced the levels of granulocytes and extrathymic T cells in various organs of mice with preexisting granulocytosis. These results suggest that some drugs have the ability to exert paradoxical effects, depending on the conditions of the host immune system. The underlying mechanisms are discussed. 続きを見る
6.

論文(リポジトリ)

論文(リポジトリ)
今井, 邦英
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  120  pp.314-323,  2006-06.  新潟医学会
本文リンク: http://hdl.handle.net/10191/5299
概要: Mice were injected with 10^4 parasitized (a nonlethal strain of Plasmodium yoelii) erythrocytes and parasitemia was enum erated in the blood. Parasitemia appeared from day 5 to day 28, showing a peak at day 18. At this peak time, lymphocytes were isolated from the brain, liver, spleen and blood. The number of lymphocyte yielded by these organs increased prominently. The phenotype of lymphocytes was examined by immunofluorescence tests. Brain lymphocytes were a mixture of IL-2Rβ^-CD3^<high> cells (conventional T cells) and IL-2Rβ^+CD3^<int> cells (extrathymic T cells) before and after infection. This pattern was quite similar to that in the liver, the only difference being that NK1.1^+ CD3^<int> cells (i.e., NKT cells) which were abundant in the liver were absent in the brain. In the case of the spleen and blood, the major lymphocytes were conventional T cells, irrespective of infection. In parallel with the increase of brain lymphocytes during infection, histology of the brain indicated the accumulation of such cells in the tissue. In the blood, parasitemia and body temperature were inversely correlated, i.e., body temperature decreased with parasitemia, and monocytes increased in number in the nonlethal-strain infection. In case of the lethal-strain infection, granulocytes increased in number in the blood and these mice died within day 5. These results suggest that brain lymphocytes might be associated with resistance or severity of malarial infection and that the major such lymphocytes were extrathymic T cells. 続きを見る
7.

論文(リポジトリ)

論文(リポジトリ)
安保, 徹
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  108  pp.83-90,  1994-02.  新潟医学会
本文リンク: http://hdl.handle.net/10191/38057
概要: The existence of extrathymically differentiated T cells was recently demonstrated by us and other investigators. The subsequent studies revealed that the sits where such extrathymic T cells are differentiated include the hepatic sinusoids and intraepithelial sites in the intestine. Especially, extrathymic T cells generated in the liver appear even in the organs other than the liver when they are activated. They may act rationally as a surveillance system against abnormal self, and sometimes seem to induce certain autoimmune diseases when they are over-activated. In this study, we have investigated detailed sites of extrathymic T-cell differentiation in the liver and their subsequent movements by using normal mice, congenitally athymic nude mice and autoimmune MRL-lpr/lpr mice (human SLE model). 続きを見る