close
1.

論文(リポジトリ)

論文(リポジトリ)
本間, 照
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  106  pp.89-97,  1992-02.  新潟医学会
本文リンク: http://hdl.handle.net/10191/39533
概要: Immunohistological study by a monoclonal antibody against DNA polymerase α was made for the investigation of labeling in dex (LI) and proliferative zone in normal mucosa and tumors (-like lesions) of the colon and rectum (normal mucosa ; n=9, hyperplastic polyps ; n=6, tubular adenomas ; n=30 and intramucosal carcinomas ; n=3). The normal mucosa and hyperplastic polyps showed a similar distribution of proliferating cells, forming a proliferative zone which restricted to the lower portion of the crypt (up to about 200μm from the glandular base). In adenomas, the distribution of proliferating cells differed by size ; small tubular adenomas, 5mm or less in size, had proliferative zone in the upper portion of neoplastic tubules (down to 200~400μm from the surface), oppositely to that of the non-neoplastic mucosa. While tubular adenomas more than 5mm showed even distribution of proliferative cells along the whole length of the tubules. In all three mucosal carcinomas, the distribution pattern was similar to that of the latter-group adenomas. The LIs was not significantly different between the proliferative zone of normal mucosa (60.8±6.6), and that of adenomas (small ; 64.6±1.6, larger ; 62.5±5.6). But in carcinomas, it was significantly higher (77.8±0.7). The formation of proliferative zone in small adenomas like non-neoplastic mucosa (but opposite location), suggests that they have a lower potential of proliferation. But the different distribution-patterns of proliferating cells in the larger adenomas indicates that the proliferating activity changes as adenomas grow. 続きを見る
2.

論文(リポジトリ)

論文(リポジトリ)
Toba, Ken ; Kishi, Kenji ; Koike, Tadashi ; Winton, Elliott F. ; Takahashi, Hoyu ; Nagai, Koichi ; Maruyama, Soichi ; Furukawa, Tatsuo ; Hashimoto, Shigeo ; Masuko, Masayoshi ; Uesugi, Yumiko ; Kuroha, Takashi ; Tsukada, Nobuhiro ; Shibata, Akira
出版情報: Experimental Hematology = Experimental Hematology.  24  pp.894-901,  1996-08.  Elsevier
本文リンク: http://hdl.handle.net/10191/1164
概要: We analyzed the surface phenotypes and cell cycle of twenty two hemopoietic cell lines using 7AAD/PY based on the cellul ar DNA/RNA content. Populations of G1a, G1b, S, and G2+M, DNA index (DI), and the RNA index of S phase (SRI) were calculated by means of DNA/RNA dot plots. Two new parameters were extracted from the cell cycle profiles, namely, the nucleic acid index of the S phase (NI) and the coefficient of variations in the RNA at S phase (SCV). DNA/RNA dot plots of cell lines revealed four characteristic profiles of the cell cycle defined with the calculated NI and SCV. These were Type 0 (small NI, large SCV), Type I (small NI, small SCV), Type II (large NI, small SCV) and Type III (large NI, large SCV). Type 0 included four stem cell lines: a t(l;19) leukemia, two Ph1 positive ALL and a biphenotypic crisis of CGL. Type I included five ALL cell lines: three T-ALL and two common B-ALL. Type II contained ten myeloid cell lines: five AML and five myeloid crisis of CGL and Type III consisted of three relatively immature lymphoma cell lines: two Burkitt's lymphoma and a follicular center lymphoma cell line. Calculated (NI / SCV(%)) were as follows: Type 0 2.27±0.19 / 16.7±3.7; Type I 2.20±0.30 / 11.1±0.7; Type II 3.64±0.52 / 11.8±1.0 and Type III 3.60±0.53 / 17.5±1.9. Cell cycle analysis of blasts using 7AAD/PY combined with surface phenotyping may yield important and rapid information for the classification of hematopoietic malignancy within 2 hours of patient admission. 続きを見る
3.

論文(リポジトリ)

論文(リポジトリ)
Toba, Ken ; Winton, Elliott F. ; Koike, Tadashi ; Shibata, Akira
出版情報: Journal of Immunological Methods = Journal of Immunological Methods.  182  pp.193-207,  1995-02.  Elsevier
本文リンク: http://hdl.handle.net/10191/1168
概要: We developed an improved technique that permits simultaneous DNA and RNA quantitation by a flow cytofluorometry using 7- amino-actinomycin D (7AAD) and pyronin Y (PY), respectively. Detailed cell cycle analyses based upon the cellular DNA/RNA levels were performed using cells suspended in a buffer containing 0.004% saponin. This method preserved the light scattering properties of human peripheral blood cells, thus lymphocyte, monocyte and granulocyte populations could be evaluated. In addition, since 7AAD and PY exhibit red (>650 nm) and orange fluorescence (570 nm) respectively, the green fluorescence channel of the flowcytometer was reserved for surface phenotyping using FITC-conjugated antibodies. The 7AAD/PY method is applicable to the simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation when combined with FITC-conjugated surface markers in heterogeneous samples. To demonstrate the three color analysis, PHA-activated human peripheral blood lymphocytes were stained for cell surface markers with monoclonal antibodies. The cells were suspended in buffer containing 0.004% saponin, then stained with 7AAD and PY. The DNA and RNA were analyzed in indivisual CD4+, CD8+ and CD20+ cells, and the characteristic cell cycle status was found. Cell activation was further analyzed using antibodies against IL-2 receptors (CD25), transferrin receptors (CD71) or HLA-DR molecules. Transferrin receptors were expressed in late G1 phase (G1B) just before the initiation of DNA synthesis, whereas IL-2 receptors and HLA-DR were expressed very early in the G1 phase (G1T). Since this technique preserves both light scatter properties as well as cell surface proteins, it is ideally suited for detailed cell cycle analyses of heterogeneous samples such as peripheral blood or bone marrow cells. 続きを見る