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論文(リポジトリ)
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内藤, 眞 ; 高塚, 尚和 ; 長谷川, 剛 ; 伊藤, 重雄 ; 児玉, 龍彦
概要:
In order to clarify the expression and tissue distribution of scavenger receptors, we have performed immunohistochemical and immunoelectron microscopical studies as well as in situ hybridization. Scavenger receptors are
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a trimeric glycoprotein mediating endocytosis of chemically modified low density lipoproteins (LDL). The receptors were detected specifically in macrophages in various tissues by immunohistochemistry using anti-bovine scavenger receptor monoclonal antibody IgG-D2, although hepatic sinusoidal cells are known to incorporate modified LDL actively. In contrast, scavenger receptor protein was detected both in Kupffer cells and endothelial cells of the hepatic sinusoid and lymphatic sinus by immunohistochemistry using anti-bovine scavenger receptor antibody IgG-D1 and anti-murine scavenger receptor antibody 2F8. In situ hybridization demonstrated the expression of scavenger receptor mRNA in Kupffer cells and sinusoidal endothelial cells. Scavenger receptors were not expressed in endothelial cells of other tissues than the liver and lymph nodes. These findings indicate that scavenger receptors are expressed both in macrophages and specific endothelial cells and that endothelial cells in various tissues constitute heterogeneous populations with regard to lipoprotein metabolism.
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| 2.
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論文(リポジトリ)
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阿部, 学
概要:
NMDA receptors are critical for synaptic plasticity and neuronal development. They are complexes consisting of the GluRζ
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1 (NR 1) subunits and one or more GluRε (ε 1-4 or NR 2 A-D) subunits. Although their synaptic localization is physiologically important in neural activities, the mechanism underlying synaptic targeting, subcellular localization, and cell surface expression remains largely unknown. To understand the synaptic localization of the NMDA receptor in cerebellum, I generated mutant mice lacking both GluRε1and ε3subunits, and made immunohistochemical and biochemical analysis in the cerebellum. There was little immunoreactivity for the GluR g 1 subunit in the granular layer of GluRε 1/ε3 mutant cerebellum, while the GluRζ1, ε1, and ε3 subunits were found to be localized at the cerebellar glomerulus in wildtype mouse. The amount of GluRζ1 protein in the GluRε1/ε3 mutant cerebellum was greatly reduced and hardly enriched in PSD fraction. These results indicate that the GluR e subunits serve as determinants of synaptic localization and protein stabilization of the GluRζ1 subunit in vivo.
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| 3.
論文(リポジトリ) |
論文(リポジトリ)
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鷲山, 和雄
概要:
Placental alkaline phosphatase (PLAP) in cases of primary intracranial germinoma was investigated by the immunohistochem
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istry and the enzyme immunoassay (EIA). On immunohistochemical examination, positive staining for PLAP was observed in neoplastic cells in all of the 15 cases examined, while the positive to negative cell ratio varied from 2 to 90%. No positive staining was observed in 97 primary intracranial nongerm cell neoplasms, but staining was observed in one of the 3 cancer metastasis cases. EIA analysis revealed intracranial PLAP levels in cerebrospinal fluid (CSF) exceeding the detection limit (20 mU/L) in 12 of the 15 germinoma patients. The range of PLAP levels in these patients was 25～1400 mU/L. Approximate, but not absolute correlation was seen between CSF PLAP levels and immunostaining of tissue sections. On the other hand, serum PLAP levels of all but one patients were under the normal cut-off level (60 mU/L). In 70 patients with primary intracranial various diseases except germ cell tumors, PLAP levels in CSF were under the detection limit. From these findings it was shown that PLAP could be used as a very useful marker for clinical and pathological diagnosis and further analysis of primary intracranial germinoma.
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| 4.
論文(リポジトリ) |
論文(リポジトリ)
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田村, 哲郎
概要:
In order to investigate the pseudocapsule, histological and immunohistochemical studies were performed on adenomas and pseudocapsules or periadenomatous pituitary glands obtained from 61 pituitary operations (acromegaly 34,
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prolactinoma 19, Cushing's disease 8). The pseudocapsule was observed in 41 cases (67.2%). The incidence was higher in acromegaly (26/34, 76.5%) than prolactinoma (13/19, 68.4%) and Cushing's disease (2/8, 25.0%). Histologically, the pseudocapsule was the tissue composed of the compressed alveolar structure with fibrosis. Immunohistochemistry demonstrated that many kinds of anterior pituitary hormone positive cells were scattered in the pseudocapsule. Some pseudocapsule (9/41, 22.0%) partly contained adenoma cells. From these observations, it is concluded that the pseudocapsule is mainly composed of normal pituitary gland compressed by the tumor. Pseudocapsulectomy after selective adenomectomy is recommended to achieve endocrinological cure in the treatment of the functioning pituitary adenomas.
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| 5.
論文(リポジトリ) |
論文(リポジトリ)
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関原, 芳夫
概要:
The effect of hyperthermia on normal brain tissues and cerebrovascular permeability have recently been reported, but its
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critical temerature is not yet well determined. The present study was aimed at the effects by two different methods, whole body and RF interstitial heating. Whole body heatings of mongrel dogs were performed with extracorporeal systemic circulation method. The temperature of the brain was continously monitored. The permeability was assessed by injection of 10% fluorescein(FL.) and/or 2% Evans blue (EB) 30 min before sacrifice, followed fluorescence photograph or visual inspection of EB extravasation. Then, using paraffin embedded formalin fixed tissues, immunohistochemical demonstration of the animal's own serum albumin and fibrinogen using the ABC method was performed. No regions in the brain heated at 43℃ for 30 or 60 min showed FL. nor EB extravasation except blood-brain barrier lacking regions and a thermosensor penetrated Site. Immunohistochemical study also showed no abnormal extravasation of serum albumin nor fibrinogen. At the same heating conditions, no histological changes were seen. RF interstitial heatings of mongrel dogs were performed as follows. A RF antenna was inserted into the brain to a depth of 10 mm from the brain surface, and were also done 4 themosensors around the antenna. Monitoring a temperature at a reference point 5～7 mm distent from the antenna, the brain was heated at 42～45℃ for 60 min. The permeability was evaluated by tracer method using EB and immunohistochemistry as described in whole body heating. A 60 min heat treatment produced a round lesion that consisted of central coagulation necrosis surrounded by a well demarcated blue zone which was heated over 44℃. The blue zone dissappeared, and 24 hrs after the treatment, changed into a coagulative/liquefactive necrosis. The vascular pemeability and histological changes were also examined at various intervals from 1 to 30 days. On 7 and 9 days after treatment, faint EB extravasation was temporarily observed at the marginal zone of the necrosis. However, in regions heated at 42～44℃, no changes of vascular permeability nor histological damages without edema were observed. These studies suggest that cerebrovascular permeability is increased over 44℃ for 60 min, then, this permeable area is changed into necrotic lesion during 24 hrs afer the treatment. However, the brain can withstand approximately at temperatures of less than 43.5℃ without changes of vascular permeability nor histological damage.
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| 6.
論文(リポジトリ) |
論文(リポジトリ)
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伊藤, 雅章
概要:
Recent advances in keratin biology were summarized and the usefulness of keratin staining and keratin electrophoresis in dermatology was introduced. Anti-hair keratin monoclonal antibodies were produced against human hair keratin filament
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proteins by mouse hybridoma method. Some of the antibodies showed immunohistochemical reactivities limited within hair tissue and others cross-reacted with hair tissue and other epithelia. The innermost cell layer of the outer root sheath in anagen hair follicle was tmmunohistochemically and ultrastructurally found to be a unique layer showing own cell differentiation. When these monoclenal antibodies and anti-soft keratin monoclonal antibodies were applied on immunohistochemical surveys of various kinds of skin epithelial tumors, the tumors were classified into several types based on their keratin expression. The keratin staining was also useful to investigate the keratinization disorder in a congenital bullous disease. As well as keratins, keratin-related substances such as keratohyalin and trichohyalin may be important for further studies in keratin biology.
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| 7.
論文(リポジトリ) |
論文(リポジトリ)
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石原, 法子
概要:
New cell lines (ECC10 and ECC12) derived from endocrine cell carcinoma (ECC) or small cell carcinoma (SCC) of the stomach were established and characterized. Primary tumor of ECC10 was an early carcinoma composed of ECC with squamous cell
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carcinoma (SQCC) and of well differentiated tubular adenocarcinoma, while that of ECC12 was an advanced carcinoma solely composed of ECC. Inoculation tumor of ECC10 was hepatic metastatic tumor with ECC and SQCC and that of ECC12 was skin metastatic tumor with ECC. ECC10 grew as both of adherent and floating aggregates and ECC12 grew as adherent aggregates. Both cell lines showed aneuploidy, about 46 hours of population doubling time, and successful xenotransplantation into athymic nude mice. ECC10 and ECC12 were composed of argyrophil cells with constant neurosecretory granules. Immunohistochemically ECC10 was negative for serotonin, peptide hormones, and chromogranin A. But, ECC12 contained some cells positive for serotonin, peptide YY, neuropeptide Y, and chromogranin A. Neuron specific enolase was positive in both cell lines, but carcinoembrionic antigen was negative. ECC10 and ECC12 still retain the characteristics of ECC components of their parent tumors and share many properties with classic type of pulmonary SCC cell lines. They are the first gastric ECC cell lines and can provide unique tools for elucidating the biological properties of gastric ECC.
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| 8.
論文(リポジトリ) |
論文(リポジトリ)
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星野, 嘉恵子 ; 周, 宜興 ; 車田, 正男
概要:
The lateral posterior complex (LP) of the thalamus receives a variety of different inputs from cortical and subcortical
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areas. We have previously demonstrated four types of vesicle containing profiles in this complex, and have identified GABA-ergic terminals. In this study, we examined the features by the light and electron microscopy of the immunoreactivity with antibodies raised against aspartate (Asp), glutamate (Glu), GABA, choline acetyltransferase (ChAT) and substance P (SP) in the LP complex. The following observations were found：(1) Asp and Glu；immunolabeled neurons were medium sized. Immunolabeled terminals were medium sized containing round vesicles and making asymmetrical synaptic contacts with dendritic profiles. (2) GABA；immunolabeled neurons were small. Immunolabeled terminals were small, contained pleomorphic synaptic vesicles and forming symmetrical synaptic contacts with dendritic profiles. (3) ChAT；no ChAT immunolabeled neurons were found. Immunolabeled terminals were small, and contained round vesicles making en passant, symmetrical and asymmetrical synaptic contacts with dendritic profiles. (4) SP；immunolabeled neurons were medium sized. Immunolabeled terminals were small containing round vesicles, and asymmetrical synaptic contacts with dendritic profile.
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| 9.
論文(リポジトリ) |
論文(リポジトリ)
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竹重, 量子
概要:
Involucrin and filaggrin are known to be cytologic markers for epidermal differentiation. Their expressions in the norma
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l skin and various types of viral warts were examined using an immunoperoxidase technique. As controls seborrheic keratosis and Bowen's disease were also examined. Two different staining patterns for involucrin were observed in viral warts; verruca vulgaris, verruca plana and condyloma acuminatum showed a homogenous staining pattern, whereas bowenoid papulosis, inclusion warts and epidermodysplasia verruciformis showed a mosaic staining pattern. It was noticeable that the involucrin expression of Bowen's disease was very similar to that of bowenoid papulosis, and that seborrheic keratosis showed a very different pattern from those of viral warts. Filaggrin staining was positive in the granular cell layer and the lower part of keratinized layers in viral warts as well as in the normal epidermis, and the pattern and intensity of staining for filaggrin were the same in both. In conclusion, although filaggrin expression does not seem to be affected in viral warts, involucrin expression is changed to some distinct patterns according to the types of viral warts. The patterns seem to be related to the proliferation activities of the affected epidermis of the viral warts.<br />ウイルス性疣贅の角化様式を明らかにするために抗involucrin抗体と抗filaggrin抗体の免疫組織化学法を行った. 正常表皮, 脂漏性角化症とポ-エン病を対照とした．Involucrinの分布は，疣贅の各型で2つパターンを示した. すなわち, 尋常性疣贅, 扁平疣贅と尖圭コンジロームの過形成表皮の細胞質内に均一に染色される所見と封入体疣贅，疣贅状表皮発育異常症とbowenoid papulosisにみられるモザイク様染色所見である．特にbowenoid papulosisのモザイク様染色はポ- エン病のそれに類似していた.．また，脂漏性角化症は疣贅各型とは異なる染色性を示した．filaggrinは疣贅各型とも，正常表皮，脂漏性角化症，ボーエン病と同様の分布および染色性で，顆粒層および一部角層に出現した．以上より，involucrinとfilaggrinはいずれも角化のマーカーであるが，特にinvolucrinの発現は，ウイルス性疣贅の各病型別にみられる角化異常に応じた変化を示すと言える．その変化は表皮細胞の分化の程度と関連があると思われた．
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| 10.
論文(リポジトリ) |
論文(リポジトリ)
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島田, 久基
概要:
Immunohistochemical distribution of laminin was examined in human renal and placental tissue using anti-human-laminin mo
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noclonal antibodies （1D8 and 6G5）, which recognized different parts of non-reduced laminin molecule. Both antibodies staind glomerular mesangial matrix and placental trophoblast basement membrane, however, glomerular basement membrane, Bowman's capsule, and tubular basement membrane were not stained. Fetal vessel wall in placenta had only limited reactivity with these two antibodies. Both tissue reduction and oxidation enhanced the reactivity of basement membrane to 1D8 but not that to 6G5. Expanded mesangial matrix in patients with diabetic nephropathy or IgA nephropathy were also stained with 1D8 and 6G5. These data suggest that laminin in extracellular matrix has immunological heterogeniety, which may be derived from masking of lammin epitopes by other matrix components or from heterogeniety of laminin chain itself.
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