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1.

論文(リポジトリ)

論文(リポジトリ)
阿部, 学
出版情報: 新潟医学会雑誌 — 新潟医学会雑誌.  115  pp.464-475,  2001-09.  新潟医学会
本文リンク: http://hdl.handle.net/10191/48383
概要: NMDA receptors are critical for synaptic plasticity and neuronal development. They are complexes consisting of the GluRζ 1 (NR 1) subunits and one or more GluRε (ε 1-4 or NR 2 A-D) subunits. Although their synaptic localization is physiologically important in neural activities, the mechanism underlying synaptic targeting, subcellular localization, and cell surface expression remains largely unknown. To understand the synaptic localization of the NMDA receptor in cerebellum, I generated mutant mice lacking both GluRε1and ε3subunits, and made immunohistochemical and biochemical analysis in the cerebellum. There was little immunoreactivity for the GluR g 1 subunit in the granular layer of GluRε 1/ε3 mutant cerebellum, while the GluRζ1, ε1, and ε3 subunits were found to be localized at the cerebellar glomerulus in wildtype mouse. The amount of GluRζ1 protein in the GluRε1/ε3 mutant cerebellum was greatly reduced and hardly enriched in PSD fraction. These results indicate that the GluR e subunits serve as determinants of synaptic localization and protein stabilization of the GluRζ1 subunit in vivo. 続きを見る
2.

論文(リポジトリ)

論文(リポジトリ)
丸山, 智宏 ; 若井, 俊文 ; 金子, 和弘 ; 大橋, 優智 ; 島田, 能史 ; 白井, 良夫 ; 畠山, 勝義
出版情報: 新潟医学会雑誌 — 新潟医学会雑誌.  122  pp.453-457,  2008-08.  新潟医学会
本文リンク: http://hdl.handle.net/10191/27973
概要: 症例は45歳男性. 健診の腹部エコーで左上腹部腫瘤を指摘され, 近医を受診し, 膵尾部腫瘍の診断で当科紹介入院となった. CT, MRI検査で膵solid-pseudopapillary tumor(以下, SPT)の診断で, 脾合併膵体尾 部切除術を施行した. 腫瘍割面は自色調の充実性部分と嚢胞性部分を認め, 嚢胞性部分には出血壊死を認めた. 組織学的には充実性部分と血管を軸とした偽乳頭構造で構成されており, SPTに合致する所見であった. 周囲脂肪組織への浸潤を認め, 組織学的にはmalignant potentialを有する腫瘍と考えられた. 免疫組織化学検査ではCD10, CD56, NSE, Vimentin, α1-antitrypsin, Chromogranin Aが陽性であり, 多方向に分化を示す腫瘍と考えられた. 膵SPTは若年女性に好発することが知られているが, 近年男性例の報告も散見される. 膵嚢胞性腫瘍の鑑別診断の際には本疾患は男性にも発症しうることを念頭に置くべきである. 本疾患は術後再発や原病死がまれなことから低悪性度腫瘍の位置づげであり, 治療の原則は外科切除である. 続きを見る
3.

論文(リポジトリ)

論文(リポジトリ)
内藤, 眞 ; 高塚, 尚和 ; 長谷川, 剛 ; 伊藤, 重雄 ; 児玉, 龍彦
出版情報: 新潟医学会雑誌 — 新潟医学会雑誌.  110  pp.216-227,  1996-06.  新潟医学会
本文リンク: http://hdl.handle.net/10191/43115
概要: In order to clarify the expression and tissue distribution of scavenger receptors, we have performed immunohistochemical and immunoelectron microscopical studies as well as in situ hybridization. Scavenger receptors are a trimeric glycoprotein mediating endocytosis of chemically modified low density lipoproteins (LDL). The receptors were detected specifically in macrophages in various tissues by immunohistochemistry using anti-bovine scavenger receptor monoclonal antibody IgG-D2, although hepatic sinusoidal cells are known to incorporate modified LDL actively. In contrast, scavenger receptor protein was detected both in Kupffer cells and endothelial cells of the hepatic sinusoid and lymphatic sinus by immunohistochemistry using anti-bovine scavenger receptor antibody IgG-D1 and anti-murine scavenger receptor antibody 2F8. In situ hybridization demonstrated the expression of scavenger receptor mRNA in Kupffer cells and sinusoidal endothelial cells. Scavenger receptors were not expressed in endothelial cells of other tissues than the liver and lymph nodes. These findings indicate that scavenger receptors are expressed both in macrophages and specific endothelial cells and that endothelial cells in various tissues constitute heterogeneous populations with regard to lipoprotein metabolism. 続きを見る
4.

論文(リポジトリ)

論文(リポジトリ)
伊藤, 雅章
出版情報: 新潟医学会雑誌 — 新潟医学会雑誌.  106  pp.585-593,  1992-07.  新潟医学会
本文リンク: http://hdl.handle.net/10191/39835
概要: Recent advances in keratin biology were summarized and the usefulness of keratin staining and keratin electrophoresis in dermatology was introduced. Anti-hair keratin monoclonal antibodies were produced against human hair keratin filament proteins by mouse hybridoma method. Some of the antibodies showed immunohistochemical reactivities limited within hair tissue and others cross-reacted with hair tissue and other epithelia. The innermost cell layer of the outer root sheath in anagen hair follicle was tmmunohistochemically and ultrastructurally found to be a unique layer showing own cell differentiation. When these monoclenal antibodies and anti-soft keratin monoclonal antibodies were applied on immunohistochemical surveys of various kinds of skin epithelial tumors, the tumors were classified into several types based on their keratin expression. The keratin staining was also useful to investigate the keratinization disorder in a congenital bullous disease. As well as keratins, keratin-related substances such as keratohyalin and trichohyalin may be important for further studies in keratin biology. 続きを見る
5.

論文(リポジトリ)

論文(リポジトリ)
石原, 法子
出版情報: 新潟医学会雑誌 — 新潟医学会雑誌.  106  pp.21-36,  1992-01.  新潟医学会
本文リンク: http://hdl.handle.net/10191/39493
概要: New cell lines (ECC10 and ECC12) derived from endocrine cell carcinoma (ECC) or small cell carcinoma (SCC) of the stomach were established and characterized. Primary tumor of ECC10 was an early carcinoma composed of ECC with squamous cell carcinoma (SQCC) and of well differentiated tubular adenocarcinoma, while that of ECC12 was an advanced carcinoma solely composed of ECC. Inoculation tumor of ECC10 was hepatic metastatic tumor with ECC and SQCC and that of ECC12 was skin metastatic tumor with ECC. ECC10 grew as both of adherent and floating aggregates and ECC12 grew as adherent aggregates. Both cell lines showed aneuploidy, about 46 hours of population doubling time, and successful xenotransplantation into athymic nude mice. ECC10 and ECC12 were composed of argyrophil cells with constant neurosecretory granules. Immunohistochemically ECC10 was negative for serotonin, peptide hormones, and chromogranin A. But, ECC12 contained some cells positive for serotonin, peptide YY, neuropeptide Y, and chromogranin A. Neuron specific enolase was positive in both cell lines, but carcinoembrionic antigen was negative. ECC10 and ECC12 still retain the characteristics of ECC components of their parent tumors and share many properties with classic type of pulmonary SCC cell lines. They are the first gastric ECC cell lines and can provide unique tools for elucidating the biological properties of gastric ECC. 続きを見る
6.

論文(リポジトリ)

論文(リポジトリ)
根本, 啓一 ; 佐藤, 啓一 ; 大西, 義久
出版情報: 新潟医学会雑誌 — 新潟医学会雑誌.  105  pp.623-628,  1991-09.  新潟医学会
本文リンク: http://hdl.handle.net/10191/39398
概要: The histopathology of the spleen of 19 patients with idiopathic thrombocytopenic purpura who underwent splenectomy was examined. The morophologic features of the spleen indicate active immune reaction: hyperplasia of lymphoid follicles with secondary germinal centers, reaction of large lymphoid cells, onion skin lesions of central arteries. Foamy histiocytes was observed in 11 of 19 cases and seroid histiocytes were found in one case. In one case, foamy histiocytes were also noted in the sinus of the lymph node. We could not clarified the relationship between the appearance and the number of foamy cells and effectiveness of splenectomy. 続きを見る
7.

論文(リポジトリ)

論文(リポジトリ)
池田, 順行 ; 星名, 秀行 ; 斎藤, 正直 ; 飯田, 明彦 ; 高木, 律男 ; 林, 孝文 ; 宇都宮, 宏子 ; 朔, 敬
出版情報: 新潟歯学会雑誌 — 新潟歯学会雑誌.  36  pp.49-53,  2006-06.  新潟歯学会
本文リンク: http://hdl.handle.net/10191/23084
概要: We report a case of granular cell tumor in the right side of the tongue. A 37-year-old man was referred to our clinic co mplaining of oppressive pain with a mass in the right side of the tongue. He found the mass three months ago. At first visit, there was a mass lesion 10x10mm in diameter with a rough surface.On ultrasonogram, the lesion with unclear borders invaded the intrinsic muscles. It was excised with a 10mm safety margin from the clinical border as is done with malignant tumors. Histopathologically, it consisted of the uniform cells which had many eosinophilic granules in their cytoplasm. In immunohistochemistry, the granules of the cytoplasm showed immunoreaction for S-100 protein, neuron-specific-enolase and cathepsinD and the margin of the cytoplasm showed immunoreaction for vimentin. Postoperatively, there has been no evidence of recurrence. The granular cell tumor is classified as a soft tissue benign tumor, however, it is very difficult to diagnose correctly from clinical appearance alone. We also compared our case with 97 other granular cell tumors that have been reported in Japan, clinico-pathologically.<br />今回われわれは、右側舌縁部に生じた顆粒細胞腫の1例を経験した。患者は37歳男性で、3ヵ月前に右側舌縁の腫瘤に気づき、接触痛が出現した。初診時、右側舌縁に表面粗造な10×10mmの腫瘤を認め、超音波検査で腫瘤は境界が不明瞭で深部は筋層に達していた。舌腫瘍の臨床診断のもと安全域を10mm設けた切除を行った。病理組織学的には、好酸性顆粒をもつ細胞の充実性増殖が認められ、被膜構造は明かではなかった。免疫組織化学的に、腫瘍細胞の細胞質内顆粒がS-100蛋白質、神経特異エノラーゼ、カテプシンD陽性を示し、細胞質辺縁部がビメンチン陽性であったことから、顆粒細胞腫と診断された。術後,再発はなく経過は良好である。顆粒細胞腫は身体各部の軟組織に発生する比較的まれな良性腫瘍であるが、その鑑別診断には注意を要する。今回、われわれは、本邦での顆粒細胞腫の報告97例を検索したので、臨床病理学的に考察をつけ加えた。 続きを見る
8.

論文(リポジトリ)

論文(リポジトリ)
大島, 勇人
出版情報: 新潟歯学会雑誌 — 新潟歯学会雑誌.  34  pp.1-13,  2004-12.  新潟歯学会
本文リンク: http://hdl.handle.net/10191/23325
概要: Regeneration-the creation of a new tissue after the original one has been lost-is the fundamental biological capability in an organism. Numerous organs are considered to contain stem cells referred to as adult stem cells, even in the adult. Adult stem cells can give rise to a limited set of adult tissue types. In the field of clinical dentistry, it is well-known that the dentin-pulp complex is capable of repair after tooth injuries such as tooth replantation/transplantation or restorative procedures including cavity preparation. This phenomenon may indicate that dental pulp stem cells exist in the pulp tissue of the matured tooth. However, the exact origin of the cells responsible for secretion of reparative dentin matrix has not been clearly identified. The existence of the dental pulp stem cells in the human wisdom or deciduous teeth, which has been reported by the recent studies, would be informative for the regenerative treatment of teeth. This review focuses on the repair responses of dental pulp to tooth injury and the possible role of antigen-presenting cells and heat shock proteins (HSPs) in the reparative processes. Moreover, attention is focused on adult stem cells in the pulp tissue. HSPs are expressed in normal various cells as well as under stressful conditions, although they were first discovered under the latter conditions. These proteins have been reported to possess diferent functions including molecular chaperones or a general mediator of inflammation. Our recent studies have demonstrated that the intense HSP-25-immunoreactivity is found in the differentiated odontoblasts. Tooth injuries such as cavity preparation or tooth replantation cause the degeneration of the odontoblast layer to result in the loss of HSP-25-immunoreactions in the suffered dental pulp. Numerous class Ⅱ major histocompatibility complex (MHC)-positive cells appeared temporarily along the pulp-dentin border after these injuries. Subsequently, newly differentiated odontoblasts acquire an HSP-25-immunoreactivity. These findings indicate that the time course of changes in the expression of HSP-25-immunoreactivity reflects the degeneration/regeneration process of odontoblasts and that the temporal appearance of the class ⅡMHC-positive cells at the pulp-dentin border is suggestive of their participation in odontoblast differentiation as well as in initial defense reactions during the pulpal regeneration process. Thus, it is important to recognize that a variety of cellular signaling from these components may be present in the extracellular milieu at sites of injury in the pulp tissue.<br />生物のもつ最も生命らしい現象の一つに再生がある。私たちのからだは、外傷や切断などの物理的損傷に対しての治癒能力を備えており、その傷を受けた場所に応じて修復し、元通りに再生する。この様な再生現象において、細胞が作り出されるかなめの部分には組織幹細胞が存在する。歯科領域においでも再生現象が知られており、窩洞形成や歯の再植・移植等の歯の損傷に対して、歯髄は再生能力を有している。しかしながら、歯髄組織再生に必要な組織幹細胞の存在は臨床経験から推察されているものの実験によっては実証されていないのが現状であり、再生の場が大きく失われると再生が期待できない場合が多い。 最近、ヒトの智歯や脱落乳歯から歯髄幹細胞を同定したという報告が相次ぎ、歯髄の再生医療は手の届きそうな段階まできたかの印象を受ける。本稿では、これまで私たちが明らかにした研究データを基盤に、歯の損傷後の歯髄修復過程における抗原提示細胞とストレスタンパク質の役割について概説し、歯髄における組織幹細胞の存在と役割についても言及する。ストレスタンパク質(熱ショックタンパク質)heat shock protein(HSP)とは、生物が高温などのストレスにさらされた時に一時的に合成が誘発されるタンパク質で、ストレスによる損傷からの自身の防御と修復に関与するが、炎症反応を活性化することも知られている。この様なストレスタンパク質のうち低分子量のHSP-25が象牙芽細胞に高濃度に存在している。窩洞形成・歯の再植後の歯髄修復過程においても、再生象牙芽細胞がHSP-25発現を示すことが明らかになっており、歯髄間葉細胞の象牙芽細胞への最終分化にストレスタンパク質が重要な役割を果たすとともに、変性した象牙芽細胞から漏出したストレスタンパク質が免疫反応に影響を与えていることが推測された。一方、この様な歯髄修復過程において、歯髄・象牙質界面にクラスⅡ主要組織適合複合体(major histocompatibility complex:MHC)分子をもつ抗原提示細胞が一過性に出現することも明らかになっている。ストレスタンパク質と抗原提示細胞の相互作用が歯髄侵襲後の迅速な象牙芽細胞分化に一役を担っているのかもしれない。歯髄の再生過程は、上皮組織が存在しない環境下で、象牙質を含む細胞外基質、免疫担当細胞の遊走、象牙芽細胞の変性という3つの側面から現象を捉える必要がある。 続きを見る
9.

論文(リポジトリ)

論文(リポジトリ)
竹重, 量子
出版情報: 新潟医学会雑誌 — 新潟医学会雑誌.  104  pp.946-959,  1990-11.  新潟医学会
本文リンク: http://hdl.handle.net/10191/35091
概要: Involucrin and filaggrin are known to be cytologic markers for epidermal differentiation. Their expressions in the norma l skin and various types of viral warts were examined using an immunoperoxidase technique. As controls seborrheic keratosis and Bowen's disease were also examined. Two different staining patterns for involucrin were observed in viral warts; verruca vulgaris, verruca plana and condyloma acuminatum showed a homogenous staining pattern, whereas bowenoid papulosis, inclusion warts and epidermodysplasia verruciformis showed a mosaic staining pattern. It was noticeable that the involucrin expression of Bowen's disease was very similar to that of bowenoid papulosis, and that seborrheic keratosis showed a very different pattern from those of viral warts. Filaggrin staining was positive in the granular cell layer and the lower part of keratinized layers in viral warts as well as in the normal epidermis, and the pattern and intensity of staining for filaggrin were the same in both. In conclusion, although filaggrin expression does not seem to be affected in viral warts, involucrin expression is changed to some distinct patterns according to the types of viral warts. The patterns seem to be related to the proliferation activities of the affected epidermis of the viral warts.<br />ウイルス性疣贅の角化様式を明らかにするために抗involucrin抗体と抗filaggrin抗体の免疫組織化学法を行った. 正常表皮, 脂漏性角化症とポ-エン病を対照とした.Involucrinの分布は,疣贅の各型で2つパターンを示した. すなわち, 尋常性疣贅, 扁平疣贅と尖圭コンジロームの過形成表皮の細胞質内に均一に染色される所見と封入体疣贅,疣贅状表皮発育異常症とbowenoid papulosisにみられるモザイク様染色所見である.特にbowenoid papulosisのモザイク様染色はポ- エン病のそれに類似していた..また,脂漏性角化症は疣贅各型とは異なる染色性を示した.filaggrinは疣贅各型とも,正常表皮,脂漏性角化症,ボーエン病と同様の分布および染色性で,顆粒層および一部角層に出現した.以上より,involucrinとfilaggrinはいずれも角化のマーカーであるが,特にinvolucrinの発現は,ウイルス性疣贅の各病型別にみられる角化異常に応じた変化を示すと言える.その変化は表皮細胞の分化の程度と関連があると思われた. 続きを見る
10.

論文(リポジトリ)

論文(リポジトリ)
勝見, 伸也
出版情報: 新潟医学会雑誌 — 新潟医学会雑誌.  104  pp.374-380,  1990-05.  新潟医学会
本文リンク: http://hdl.handle.net/10191/34072
概要: To investigate diagnostic value of carcinombryonic antigen (CEA) staining in sweat gland tumors, the normal skin and several kinds of epithelial skin tumors were immunohistochemically examined by the immunoperoxidase technique using polyclonal anti-CEA antibodies (PCEA) and monoclonal anti-CEA antibody (MCEA). In the normal skin tissue, the secretory cells, ductal cells, duct cuticle and luminal materials of either eccrine glands or apocrine glands showed PCEA-positive reactions. None of the other skin components showed positive reactions to the PCEA. A weak MCEA-positive reaction was seen only in the dermal and intraepidermal portions of the sweat gland ducts. In the skin tumors, PCEA positive reactions were found in all the cases of extramammary Paget's disease and sweat gland tumors. In all the cases of the squamous cell carcinoma and Bowen's disease, the keratotic parts showed PCEA-positive reactions. These reactions became very weak or negative, when stained with MCEA. Most of the PCEA-positive materials in the skin tissues may be different from the original CEA. Although PCEA is still thought to be important as an immunohistochemical marker for the diagnosis of sweat gland tumors, there may be a heterogeneity of PCEA-reacting substances among normal skin and tumor tissues. 続きを見る