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1.

論文(リポジトリ)

論文(リポジトリ)
山本, 洋子
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  112  pp.552-562,  1998-09.  新潟医学会
本文リンク: http://hdl.handle.net/10191/46200
概要: To investigate the effect of epidermal growth factor (EGF) on cell migration of squamous cell carcinoma (SCC) cells, mig ration, adhesion and spreading activity, organization of focal adhesion and actin stress fiber, integrin expression, and tyrosin phosphorylation of focal adhesion kinase (FAK) of a cell line were examined on type IV collagen in the presence of various concentrations of EGF. The cell line that was separated from an established cell line of SCC in uterine cervix, CaSki, expresses α2, α3, α6, β1 and β4 integrins, and was used in this study. Wound assay demonstrated that EGF increased the migration activity at 1 and 10 ng/ml, whereas it completely blocked the migration at 100 ng/ml. Blocking antibodies against α3, α6 and β1 integrins inhibited the migration in the absence of EGF, whereas antibodies against not only these integrins but also α2 inhibited it in the presence of 10 ng/ml EGF. Flowcytometry showed that EGF increased the expression of α2 and α6 integrins in a dose dependent manner. Attachment assay revealed that EGF decreased the adhesion activity to type IV collagen in a dose dependent manner. Morphological examinations showed that EGF inhibited the spreading at 10 and 100 ng/ml, and that the growth factor did not alter the organization of focal adhesions and actin stress fibers at 1 and 10 ng/ml, whereas it increased it at 100 ng/ml. Immunoprecipitation and immunoblot assays showed that EGF did not alter tyrosin phosphorylation level of FAK at any concentrations examined. These findings suggest that the EGF-mediated promotion of the migration at 1 and 10 ng/ml is related with the modulation of α2 integrin function and expression, and the inhibition of the adhesion, whereas EGF-mediated inhibition of the migration is related with the modulation of organization of actin stress fiber and focal adhesion, and that alteration in a tyrosin phosphorylation level of FAK is not always accompanied with an increase in the migration activity. 続きを見る
2.

論文(リポジトリ)

論文(リポジトリ)
兼子, 泰行
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  105  pp.177-186,  1991-03.  新潟医学会
本文リンク: http://hdl.handle.net/10191/38651
概要: To investigate alterations in the basement membrane (BM) and of fibronectin receptor (FNR) in malignant melanoma (MM), t he tumor were studied immunohistochemically by double immunofluorescent staining using monoclonal antibodies to the core protein of heparan sulfate proteoglycan(HSPG), collagen type III(C-3)and chondroitin 6-sulfate (C6S) as well as antiserum to laminin, collagen type IV (C-4), fibronectin and fibronectin receptor. In MM in situ, C-4, laminin and HSPG were preserved as a continuous linear band on the dermo-epidermal junction (DEJ), while C6S is extensively lost from the DEJ and fibronectin is remarkably decreased in the papillary dermis compared with the normal skin. In invasive, MM, although the distribution pattern was varied case by case, the BM components were not decreased. The cases were divided into two groups by the distribution pattern; a great amount of each BM component were localized on the surface of tumor cells, or surrounded tumor nests, forming the large nests. In all but one cases examined, however, C6S was absent from the BM zone of the melanoma nests in the dermis. These data indicates that although C6S was absent, the other BM components were actively synthesized by tumor cells, and that the BM components were altered in phase of MM in situ. Intensity of FNR of melanoma cells were generally more fainter than those of infiltrating lymphocytes in the dermis, basal cells of the epidermis and endothelial cells of small vessels. There were no significant differences in their intensity of melanoma cells between each tumor progression phase. Therefore, the present study may reveal that these receptors do not relate to invasion or metastasis of the tumor. 続きを見る
3.

論文(リポジトリ)

論文(リポジトリ)
片貝, 智哉
出版情報: 新潟医学会雑誌 = 新潟医学会雑誌.  130  pp.269-274,  2016-05.  新潟医学会
本文リンク: http://hdl.handle.net/10191/44391
概要: 獲得免疫応答の中枢として機能するリンパ節において, Tリンパ球は傍皮質領域に集積し, 毎分平均10μm以上という非常に速い速度で活発に遊走している. この現象は, 末梢組織からリンパ管経由で移動してきた樹状細胞が提示する抗原を効率良く探索し , 効果的な免疫応答を誘導するために必要であると推測される. 近年の多光子励起レーザー顕微鏡を用いた生体イメージングによりそのメカニズムに関する解析が進み, 特に樹状細胞やストローマ細胞などの組織微小環境を構成する細胞の役割が重要であることが明らかになりつつある. 本稿では, 筆者らによる最新の成果を中心にリンパ節内におけるT細胞高速間質遊走の分子機構を概説する. 続きを見る