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抗サイトケラチンモノクロナール抗体を用いた脳腫瘍の髄液診断に関する研究

フォーマット:
論文(リポジトリ)
責任表示:
河野, 充夫
出版情報:
新潟医学会, 1992-07
掲載情報:
新潟医学会雑誌 = 新潟医学会雑誌
ISSN:
00290440  CiNii Research  Webcat Plus  JAIRO
著者名:
河野, 充夫  
巻:
106
通号:
7
開始ページ:
603
終了ページ:
611
バージョン:
publisher
概要:
A new assay system for cytokeratin (CK) in cerebrospinal fluid (CSF) of patients with intracranial tumors was established. Antiboby KWS-1 was a murine monoclonal immunoglobulin generated from a fusdion of a mouse myeloma cell line with lymphocytes from a mouse immunised with the cytoskeleton from established human 1arge cell carcinoma cell line. KWS-1 was of IgG_1 subclass . KSW-1 antibody as characterized by immunoblotting identified CK subunit molecular weight at 56, 54, 52.5, 46, 45 and 40 Kd and did not cross react with other intermediate sized filament proteins. Extensive assesment by immunoperoxidase staining on formalin or ethanol fixed paraffin embedded human tissue sections showed wide reactivity in varieties of epithelial cells and tumors. An amplified sandwich assay was developed for the measurment of CK. Lower limit of antigen detection of this assay was at 50 ng/ml. CK in CSF of 57 patients with intracranial tumors and 14 non-neoplastic intracranial diseases were measured. CK levels were elevated in metastatic carcinoma (5/20), epidermoid (1/3), epidermoid carcinoma (1/1) and germ cell tumor (1/4), but under lower limit in other primary brain tumors and non-nepolastic intracranial diseases. These findings suggested that the measurement of CK in CSF may be a useful tumor marker in patients with metastatic carcinoma and some primary intracranial epithelial tumors. 続きを見る
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