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Article
Niimura, Sueo ; Kawakami, Shin-ya
Published: 新潟大学農学部研究報告 — 新潟大学農学部研究報告.  55  pp.91-97,  2003-03.  新潟大学農学部
Full Text Link: http://hdl.handle.net/10191/25112
Abstract: The activities of △^5-3β-hydroxysteroid dehydrogenase (△^5-3β-HSD) (pregnenolone and 17α-hydroxypregnenolone as the subs trates), 20α-HSD (20α-hydroxyprogesterone as the substrate) and 20β-HSD (17α-hydroxyprogesterone and 20β-hydroxyprogesterone as the substrates) were histochemically demonstrated in mouse eggs and embryos, and the progestagen production in those during the early development was examined. In unfertilized mouse eggs, the activities of such HSDs were observed in 88 to 98% of those. The percentages of embryos showing the activities of such HSDs did not change during the early development. It was also histochemically confirmed that the activity of △^5-3β HSD with DHA as the substrate, which is involved in steroid biosynthesis, was observed in 86 to 100% of the eggs and embryos from the un fertilized to blastocyst stages. From the present findings, it was suggested that the progestagens such as progesterone, 17α-hydroxyprogesterone, 17α,20β-dihydroxyprogesterone, 20α-hydroxyprogesterone and 20β-hydroxyprogesterone are constantly synthesized in mouse eggs and embryos during the early development.<br />マウスの未受精卵子および前核期から胚盤抱期までの初期胚について、△^5-3β-HSD(基質としてpregnenoloneおよび17α-hydroxypregnenoloneを使用)、 20α-HSD (20α-hydroxyprogesterone)および20β-HSD (17α-hydroxyprogesterone、 20β-hydroxyprogesterone)の活性を組織化学的に検出し、プロジェスタジェン合成能を検肘した。未受精期の卵子において、これらHSDの活性は88ないし98%のものに認められた。また、前核期から胚盤抱期までの初期胚においても、 83%以上でこれらのHSD活性が検出された。一方、ステロイド生合成の指標となる△^5-3β-HSD (DHA)の活性は、 86%以上の卵子と初期胚に常に観察された。これらのことから、発生初期のマウスの卵子と胚は、常にprogesterone、 17α-hydroxyprogesterone. 17α,20β-hydroxyprogesterone、 20α-hydroxyprogesteroneおよび20β-hydroxyprogesteroneの合成を行っており、これらプロジェスタジェンの合成能は、胚の初期発生に伴って変化しないことが考えられた。 Read more
2.

Article

Article
Niimura, Sueo ; Kawakami, Shin-ya
Published: 新潟大学農学部研究報告 — 新潟大学農学部研究報告.  55  pp.27-34,  2002-08.  新潟大学農学部
Full Text Link: http://hdl.handle.net/10191/25111
Abstract: The activities of hydroxysteroid dehydrogenases (HSDs) were histochemically demonstrated in mouse oocytes in the process of maturation in vivo and in vitro, and the changes in steroid metabolism during meiotic maturation were examined. In mouse oocytes soon after collection, the activities of △5-3β- HSD, 17β-HSD and 20β-HSD were observed in 87 to 97% of those, while the activity of 20α-HSD was not. The percentages of oocytes showing the activities of △5-3β-HSD (using DHA, pregnenolone and 17α- hydroxypregnenolone as the substrates), 17β -HSD (estradiol-17β and testosterone) and 20β -HSD (17α- hydroxyprogesterone and 20β-hydroxyprogesterone) did not change during maturation in vivo and in vitro. The oocytes with the activity of 20α-HSD (20α hydroxyprogesterone) appeared 4 hrs after the hCG injection or after culturing for 4 hrs and the rates of those reached 92 and l00%, respectively, 14 hrs after the hCG injection or after cultunng for 14 hrs. In the oocytes cultured for 8 hrs with olomoucine, an inhibitor of cychn-dependent kmase, nuclei were almost in the germinal vesicle stage, and the activity of 20α-HSD (20α hydroxyprogesterone) was observed in 84% of the treated oocytes. On the other hand, 81% of control oocytes also showed such the HSD activity, showing no difference from the rate of the treated oocytes. From the present findings, it was suggested that the metabolic abilities of progesterone, Viahydroxyprogesterone, 17α,20β dihydroxyprogesterone, 20β -hydroxyprogesterone, estradiol-17/7 and androgen are constantly present in mouse oocytes in the process of maturation in vivo and in vitlo. And it was also suggested that the metabolic ability of 20α-hydroxyprogesterone in mouse oocytes varies with maturation, while the change in the metabolic ability of such steroid is not related to nuclear maturation.<br />体内および体外で成熟過掛こあるマウス卵母細胞について、各種ヒドロキシステロイド脱水索酵素(HSD)の活性を組織化学的に検出し、卵母細胞の成熟に伴うステロイド代謝能の変化を検肘した。採取直後の卵母細胞において、 87ないし97%で△^5-3β-HSD、 17β-HSDおよび20β-HSDの活性が検出されたが、 20α-HSDの活性は検出されなかった。一方、 △^5-3β-HSD(基質としてDHA、 pregnenoloneおよび17α hydroxypregnenoloneを使用)、 17β-HSD (estradiol-17βとtestosterone)および20β-HSD O7α hydroxyprogesteroneと20β-hydroxyprogesterone)の活性を示す卵母細胞の割合は、体内および体外での成熟の経過に伴う変化を示さなかったが、成熟に伴って、20α-HSD (20α-hydroxyprogesterone)の活性を示す卵母細胞は出現し、その割合は高くなった。また、 8時間オロモウシン処置した卵母細胞において、核はほとんどで卵核胞期にあるとともに、 20α-HSD (20α-hydroxyprogesterone)の活性を示すものの割合は84%であり、オロモウシン処置していない対照の卵母細胞の81%と相違なかった。以上の結果から、マウス卵母細胞のprogesterone、 17α-hydroxyprogesterone、 17α,20β-dihydroxyprogesterone、 20β-hydroxyprogesterone、 estradiol-17βおよびandrogenの代謝能は、体内および体外での成熟に関わらず変化しないことが示唆された。また、マウス卵母細胞では、成熟に伴って20α- hydroxyprogesteroneの代謝能は出果して高まるが、この代謝能の変化は、核の成熟とは関係ないことが推察された。 Read more