Estimation of contribution of nitrogen fixation in sugarcane (Saccharum officinarum L.) plants with 15N dilution method in relation to nitrogen supply period.

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Article

Responsibility:

Hiyama, Takahiro ; Momose, Atsushi ; Nishimura, Keiko ; Ishizaki, Noriko ; Ishikawa, Shinji ; Yamamoto, Misaki ; Hung, Ngyuen Van Phi ; Chamaiporn, Anuwong ; Ruamrungsri, Soraya ; Ohtake, Norikuni ; Sueyoshi, Kuni ; Ohyama, Takuji

Published:

新潟大学農学部, 2013-10

Publication info:

新潟大学農学部研究報告 — 新潟大学農学部研究報告

ISSN:

03858634      

Authors:

Hiyama, Takahiro

Momose, Atsushi

Nishimura, Keiko

Ishizaki, Noriko

Ishikawa, Shinji

Yamamoto, Misaki

Hung, Ngyuen Van Phi

Chamaiporn, Anuwong

Ruamrungsri, Soraya

Ohtake, Norikuni

Sueyoshi, Kuni

Ohyama, Takuji

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Volume:

66

Number:

1

Start page:

11

End page:

19

Abstract:

Pot experiment was conducted in warm greenhouse in Niigata University to estimate the contribution of nitrogen fixation in sugarcane (Saccharum officinarum L. var. NiF8) plants using 15N dilution method in relation to N supply period. Sugar … cane plants were grown from a cut stalk in water for 20 days and the young shoots were transplanted to the 1/5000 a Wagner pot filled with vermiculite. Three fertilizer treatments were applied, 1) N0: Nitrogen free culture solution was supplied, 2) N100: 15N labeled ammonium sulfate was supplied at the rate of 100 mgN per pot a week, 3) N100N0: 15N labeled ammonium sulfate was supplied at the rate of 100 mgN per por a week until 6 weeks after transplanting (WAT), then the plants were cultivated without N supply. The growth of the plants was measured every week, and plants were harvested at 12 WAT and 20 WAT. Total nitrogen content and 15N abundance in each part were determined. Acetylene reduction activity was measured for each part of harvested plants. N0 plants grew very poor, and sole nitrogen fixation is not enough to support vigorous growth of sugarcane plants. N100 plants and N100N0 plants showed relatively similar shoot length and leaf number, but the N100N0 plants leaves were pale and the N concentration was almost a half of N100 plants. At 12 WAT, total N content was 408 mgN and 286 mgN per plant in N100 treatment and N100N0 treatment, respectively. The amount of nitrogen derived from nitrogen fixation (Ndfa) in N100 and N100N0 was 87 mgN (21%Ndfa) and 48 mgN (17%Ndfa) per plant respectively. At 20 WAT, total N content was 569 mgN (100N) and 292mgN (100N0N), and the amount of Ndfa was 87 mg (15%Ndfa) in 100N and 57 mgN (20%Ndfa) in 100N0N treatment. Among organs, the estimated %Ndfa tended to be higher in old leaves and stalk, and lower in green leaves and stems. From this experiment, the continuous supply of N fertilizer did not inhibit nitrogen fixation in sugarcane compared with N deficient plants.<br />施肥窒素供給期間の違いによるサトウキビ（Saccharum officinarum L., 品種農林８号) の窒素固定寄与率を15N希釈法で調べる目的で新潟大学の温室内でポット試験を実施した。サトウキビの種茎を水中で発芽させたのち、バーミキュライトを充填した1/5000 a のワグネルポットで栽培した。窒素施肥処理は、３区設けた。１) N0区: 無窒素培養液のみ供与、 ２) N100: 15N標識の硫酸アンモニウム水溶液（100mgN）を週１回ポットに添加、３) N100N0: 移植から移植後６週目まで、15N標識の硫酸アンモニウム水溶液（100mgN）を週１回ポットに添加し、その後無窒素栽培した。移植後植物の地上部の長さと葉数を毎週測定した。また、移植12週間後と20週間後に植物を採取し、各部位の窒素含有量、15N濃度とアセチレン還元活性を測定した。N0区の植物はほぼ生長が停止し、窒素固定だけでは生育を支えるには不十分であることが確認された。N100区とN100N0 区の植物は、地上部の長さや葉の数は、大きな差はなかったが、N100N0区の葉は葉色が薄く、窒素含有量もN100区の半分程度であった。移植12週間後には, 100 区とN100N0 区の株あたり全窒素含有率は、それぞれ408 mgN と286 mgN であった。15N 希釈法で推定した窒素固定量は、N100 区とN100N0 区でそれぞれ株あたり87mgN（窒素固定由来窒素割合%Ndfa, 21％）、48mgN（17%Ndfa）であった。移植20週間後では、N100区とN100N0区の株あたり全窒素含有率は、それぞれ569mgNと292mgNであった。窒素固定推定量は、N100区とN100N0区でそれぞれ株あたり87mgN（%Ndfa, 21％）、57mgN（33％ Ndfa）であった。器官別窒素固定割合では、枯葉、種茎が高く、緑葉、茎が低い傾向がみられた。 Read more

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