Blockade of CD26 Augments the Therapeutic Potential of Marrow Stem Cells in the Liver
- フォーマット:
- 論文(リポジトリ)
- 責任表示:
- Matsuda, Yasunobu ; Wakai, Toshifumi ; Hirose, Yuki ; Sakata, Jun ; Kobayashi, Takashi ; Osawa, Mami ; Fujimaki, Shun ; Kubota, Masayuki
- 出版情報:
- 新潟大学医学部保健学科, 2014-03
- 掲載情報:
- 新潟大学保健学雑誌 — 新潟大学保健学雑誌
- ISSN:
- 21884617
- 著者名:
Matsuda, Yasunobu Wakai, Toshifumi Hirose, Yuki Sakata, Jun Kobayashi, Takashi Osawa, Mami Fujimaki, Shun Kubota, Masayuki - 巻:
- 11
- 通号:
- 1
- 開始ページ:
- 39
- 終了ページ:
- 49
- 概要:
- Background & Aims: The therapeutic potential of marrow stem cells for liver disease is currently unclear. The aim of this study was to investigate whether potentiating the chemotaxis ability of stromal cell-derived factor 1 could augmen … t the engraftment of marrow stem cells and ameliorate tissue damage. Methods: Female mice reconstructed with β-galactosidase-positive male marrow cells were repeatedly injected with carbon tetrachloride to induce liver cirrhosis. The statuses of stromal cell-derived factor 1 and its inhibitor CD26 were examined by immunohistochemical staining, enzyme-linked immunosorbent assays and colorimetric assays. Recipients were treated with an inhibitory peptide for CD26(Diprotin A)together with granulocyte colony-stimulating factor, and the marrow-derived cells were tracked through the β-galactosidase activity or sex-determining region on the Y chromosome. Results: Stromal cell-derived factor 1 expression and CD26 activity were both significantly increased and marrow-derived hepatocytes were not observed in liver cirrhosis, suggesting that the potential of stem cell homing is impaired by liver damage. Granulocyte colony-stimulating factor only induced a few cell clusters comprising 2-6 marrow-derived hepatocytes at a late stage of liver cirrhosis. In contrast, when animals with liver cirrhosis were treated with Diprotin A after granulocyte colonystimulating factor-induced stem cell mobilization, marrow-derived hepatocytes increased to 2-4% of the total hepatocytes and both the serum albumin and hepatic hydroxyproline contents were ameliorated(p < .05). Conclusions: Maintaining the chemotaxis ability of stromal cell-derived factor 1 may represent an effective strategy for validating stem cell therapy. 続きを見る
- E-Resources:
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